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ca 2 free hbss  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ca 2 free hbss
    Tight junction modulation in human multicellular hepatic spheroids (MHS) on culture day 14. Donor WID MHS were incubated in standard HBSS containing 5 µM CDFDA for 30 min at 37 °C on culture day 14. MHS were washed and maintained in standard HBSS (“cells + bile”) or Ca 2+ -free HBSS (“cells only”) for up to 20 min and imaged over time using a CellInsight CX7 High Content Scanner (10 µm step size, 12 steps, 120 µm extended depth of field, 10X objective). Transmitted light (grey) was used to visualize MHS structure. White arrows depict CDF accumulation in bile pockets in standard HBSS (top panel) or CDF dispersing from bile pockets in Ca 2+ -free HBSS (bottom panel). Scale bar = 100 µm. CDFDA, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate; HBSS, Hank’s balanced salt solution
    Ca 2 Free Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca+2+free+hbss/pmc12804587-31-32-52?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    ca 2 free hbss - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "Evaluation of a human 3D multicellular hepatic spheroid model as a platform for studying hepatic transporters"

    Article Title: Evaluation of a human 3D multicellular hepatic spheroid model as a platform for studying hepatic transporters

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-025-05996-z

    Tight junction modulation in human multicellular hepatic spheroids (MHS) on culture day 14. Donor WID MHS were incubated in standard HBSS containing 5 µM CDFDA for 30 min at 37 °C on culture day 14. MHS were washed and maintained in standard HBSS (“cells + bile”) or Ca 2+ -free HBSS (“cells only”) for up to 20 min and imaged over time using a CellInsight CX7 High Content Scanner (10 µm step size, 12 steps, 120 µm extended depth of field, 10X objective). Transmitted light (grey) was used to visualize MHS structure. White arrows depict CDF accumulation in bile pockets in standard HBSS (top panel) or CDF dispersing from bile pockets in Ca 2+ -free HBSS (bottom panel). Scale bar = 100 µm. CDFDA, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate; HBSS, Hank’s balanced salt solution
    Figure Legend Snippet: Tight junction modulation in human multicellular hepatic spheroids (MHS) on culture day 14. Donor WID MHS were incubated in standard HBSS containing 5 µM CDFDA for 30 min at 37 °C on culture day 14. MHS were washed and maintained in standard HBSS (“cells + bile”) or Ca 2+ -free HBSS (“cells only”) for up to 20 min and imaged over time using a CellInsight CX7 High Content Scanner (10 µm step size, 12 steps, 120 µm extended depth of field, 10X objective). Transmitted light (grey) was used to visualize MHS structure. White arrows depict CDF accumulation in bile pockets in standard HBSS (top panel) or CDF dispersing from bile pockets in Ca 2+ -free HBSS (bottom panel). Scale bar = 100 µm. CDFDA, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate; HBSS, Hank’s balanced salt solution

    Techniques Used: Incubation



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    Image Search Results


    Tight junction modulation in human multicellular hepatic spheroids (MHS) on culture day 14. Donor WID MHS were incubated in standard HBSS containing 5 µM CDFDA for 30 min at 37 °C on culture day 14. MHS were washed and maintained in standard HBSS (“cells + bile”) or Ca 2+ -free HBSS (“cells only”) for up to 20 min and imaged over time using a CellInsight CX7 High Content Scanner (10 µm step size, 12 steps, 120 µm extended depth of field, 10X objective). Transmitted light (grey) was used to visualize MHS structure. White arrows depict CDF accumulation in bile pockets in standard HBSS (top panel) or CDF dispersing from bile pockets in Ca 2+ -free HBSS (bottom panel). Scale bar = 100 µm. CDFDA, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate; HBSS, Hank’s balanced salt solution

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Evaluation of a human 3D multicellular hepatic spheroid model as a platform for studying hepatic transporters

    doi: 10.1007/s00018-025-05996-z

    Figure Lengend Snippet: Tight junction modulation in human multicellular hepatic spheroids (MHS) on culture day 14. Donor WID MHS were incubated in standard HBSS containing 5 µM CDFDA for 30 min at 37 °C on culture day 14. MHS were washed and maintained in standard HBSS (“cells + bile”) or Ca 2+ -free HBSS (“cells only”) for up to 20 min and imaged over time using a CellInsight CX7 High Content Scanner (10 µm step size, 12 steps, 120 µm extended depth of field, 10X objective). Transmitted light (grey) was used to visualize MHS structure. White arrows depict CDF accumulation in bile pockets in standard HBSS (top panel) or CDF dispersing from bile pockets in Ca 2+ -free HBSS (bottom panel). Scale bar = 100 µm. CDFDA, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate; HBSS, Hank’s balanced salt solution

    Article Snippet: Acetonitrile (Cat. No. A995), methanol (Cat. No. A456), water (Cat. No. W6), formic acid (Cat. No. A117), phosphate-buffered saline (PBS; Cat. No. 14190), standard Hank’s balanced salt solution (HBSS; Cat. No. 14025), Ca 2+ -free HBSS (Cat. No. 14175), 5(6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA; Cat. No. C369), and qPCR TaqMan probes were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Incubation